Molecular characterization of any gene involves the analysis of both temporal and spatial distribution of RNA expression. Gene Detect TM Kits were developed to analyze the quality of cDNA synthesis/cDNA library prior to invest your valuable time, research effort, and expensive reagents on subsequent molecular manipulation studies. Our ExpressionMersTM provided in this kit will help you to detect the constitutive expression of human house keeping genes.

PERFORM A QUALITY CONTROL OF YOUR RNA/DNA

-Using our Gene DetectTM Kits you can perform various Quality Control Assays:

-Check the quality of cDNA synthesis & cDNA Library Construction.

-Measure the purity of RNA and DNA during your isolation procedures.

-It is easy to detect tube-to-tube variations in PCR.

-Run parallel PCR reactions and get an idea about Quantitative RT-PCR.

-Make your own cDNA probes for high-throughput MicroArray screening, Southern and northern hybridizations.

-Determine Comparative Gene Expression Profiles of your target gene by ‘Normalization’ with house keeping genes in a single tube.

Gene discovery methods include subtractive hybridization, differential display, and serial analysis of gene expression relied mostly on the synthesis of cDNA from RNA source. Our Gene DetectTM Kits will provide reagents for a simple housekeeping expression profiling. cDNA representation and cDNA length is crucial in the gene characterization studies. Keeping in mind the expression levels of housekeeping genes from highly abundant, moderately abundant and lower levels, we have developed several kits to choose. Selecting and choosing your ExpressionMersTM supplied in the kit will help investigators to study the human gene target levels. Generally, highly abundant genes exists at ~3,000 copies per cell, moderately abundant genes are expressed at 300-3,000 and low abundant genes at <300 i.e. they express at 1%, 0.1%-1.0%, and <0.1% of total RNA.

The best cDNAs should represent all of the genes including housekeeping genes from a mRNA collection. Our Gene DetectTM Kit contains four sets of different RT-PCR ExpressionMersTM that are used to amplify human house keeping genes. Each ExpressionMerTM set is designed to provide you a certain length of housekeeping genes as shown in the tables.

YOUR TIME IS VALUABLE-DETECT IT FIRST

In order to demonstrate the power of the Gene DetectTM Kits, we analyzed a cDNA preparation from a mammalian RNA source. In addition, we also used genomic DNA preparation and amplified the cDNA with our ExpressionMersTM supplied in the Kits and shown in Figure 1.

Lanes 2, 3, 4 and 5: Beta Actin (450 bp), Elongation factor I alpha (533 bp), 28S rRNA (482 bp) and Histone 4 (288 bp) supplied in Gene DetectTM Kit I.

Lanes 8, 9, 10 and 11: Alpha Tubulin (534 bp), ß Globin (485 bp), PhosphoLipase A2 (502 bp), and 18S rRNA (529 bp) supplied in the Gene DetectTM Kit II.

Lanes 14,15,16 and 17: GA3PDH (404 bp), ß2 Microglobulin (502 bp), Transferrin receptor (468 bp) and Cathepsin D (527 bp) supplied in the Gene DetectTM Kit III.
Lanes 1, 7,13 and 19: Molecular weight marker 100 bp DNA ladder; Lanes 6, 12 and 18: Negative control from each kit.

ORDER TODAY

· It is so simple to perform a quick check of your cDNA.

· It is easy to do a PCR with our ExpressionMersTM.

· Try one of our Gene DetectTM Kits today.

Each Gene DetectTM Kit comes with four ExpressionMerTM pairs, sterile water, and a detailed instruction manual. AmpliTaq DNA Polymerase and dNTPs are not supplied with these kits; they should be purchased separately from either PE BioSystems or any other distributor.

Each kit comes with enough reagents to conduct 50 reactions.

Why do you wait! Order the Gene DetectTM Kit Today. Receive a FREE T-Shirt.

Each pair of ExpressionMersTM will be also available separately at a price of $75.00.