AccuPower® PCR PreMix & Master Mix Series – Precision Amplification for Modern Molecular Biology

In today’s molecular diagnostics and life science research landscape, accuracy, speed, and reproducibility are everything. Whether you're performing routine gene amplification, mutation screening, cloning, pathogen detection, or multiplex assays, your PCR chemistry determines your success.

The AccuPower® PCR PreMix and Master Mix series delivers optimized, ready-to-use PCR reagents designed to streamline workflows while maximizing amplification performance.

Why Choose AccuPower® PCR Solutions?

The AccuPower® line is engineered for:

• 🔬 High specificity amplification

• ⚡ Reduced setup time

• 🧪 Improved reproducibility

• 🛡️ Contamination control options

• 🎯 Flexible enzyme choices for every application

From standard Taq reactions to high-fidelity Pfu polymerase systems, this portfolio covers virtually every PCR requirement in research and diagnostics.

Product Categories Overview

1️⃣ Standard PCR Solutions

Best for:  Routine PCR, cloning, genotyping, and academic research.

These ready-to-use formulations contain:

• Optimized reaction buffer

• dNTPs

• Taq DNA polymerase

• Stabilizers for consistent amplification

Perfect for laboratories seeking reliable, cost-effective DNA amplification.

2️⃣ Contamination-Control PCR (UDG System)

Designed to prevent carryover contamination, these mixes incorporate:

• Uracil-DNA Glycosylase (UDG)

• dUTP system compatibility

Ideal for clinical PCR labs, high-throughput diagnostic workflows, and pathogen detection platforms.

3️⃣ HotStart Technology for Superior Specificity

HotStart chemistry prevents nonspecific amplification and primer-dimer formation by keeping the polymerase inactive until the initial denaturation step.

Applications include:

• Low-copy template amplification

• SNP detection

• Sensitive mutation analysis

• Diagnostic PCR panels

The GoldHotStart and PyroHotStart formulations enhance reaction stability and performance under demanding thermal cycling conditions.

4️⃣ High-Fidelity & Proofreading PCR Systems

• AccuPower® ProFi Taq PCR PreMix / Master Mix

• AccuPower® HotStart ProFi Taq PCR PreMix

• AccuPower® Pfu PCR PreMix / Master Mix

• AccuPower® HotStart Pfu PCR PreMix

• For applications requiring enhanced fidelity and accurate DNA replication:

• Proofreading activity (3’→5’ exonuclease)

• Lower error rates than conventional Taq

• Ideal for cloning, sequencing, and gene construction

These formulations are especially suitable for:

• Expression vector construction

• Site-directed mutagenesis

• Long-fragment amplification

5️⃣ Multiplex PCR Systems

• AccuPower® Multiplex PCR PreMix / Master Mix

• AccuPower® Gold Multiplex PCR PreMix

Multiplex PCR enables simultaneous amplification of multiple targets in a single reaction.

Advantages:

• Reduced reagent cost

• Time efficiency

• Consistent multi-target amplification

• Diagnostic panel development

Ideal for:

• Genetic disorder screening

• Biomarker detection

• Forensic testing

PreMix vs. Master Mix – What’s the Difference?

Both formats simplify PCR workflows and minimize pipetting errors.