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  • What is Quantum™ R-PE MESF?

  • Quantum™ R-PE MESF is a kit of calibrated fluorescent microbeads whose intensity is expressed in MESF (Molecules of Equivalent Soluble Fluorochrome).
  • This means that each bead population corresponds to a known amount of equivalent soluble fluorochrome, enabling absolute quantification of fluorescence.
  • The fluorochrome used is:
  • R-phycoerythrin (R-PE)   widely used for cell labeling in flow cytometry.
  • Main Purpose
  • This product is used to:
  • ✔ Standardize fluorescence intensity between instruments
  • ✔ Convert cytometer signals into quantitative MESF units
  • ✔ Measure the true expression level of cellular markers
  • ✔ Verify instrument linearity and sensitivity
  • In practice, the beads are analyzed together with samples to generate a fluorescence → MESF calibration curve, which is then used to quantify cells.
  • Kit Composition
  • A typical Quantum MESF kit contains:

    • 1 population of non-fluorescent beads (blank)

    • 4 fluorescent bead populations with increasing intensity

    • Microbeads approximately 7–9 µm in diameter (similar in size to human lymphocytes)

    • Each population has a certified MESF value.

    Scientific Principle

    The system is based on direct comparison between:

    • Measured fluorescence of calibrated microbeads

    • Fluorescence of the pure fluorochrome

    • Conversion of the instrument signal into MESF

    • Result: absolute and reproducible quantification of cellular fluorescence.

    Available Formats

    Typically available in several volumes:

    • 1 mL (~20 tests)

    • 5 mL (~100 tests)

    • 14 mL (~280 tests)

    • Typical Applications

    ✔ Quantitative immunophenotyping

    ✔ Antigen expression analysis

    ✔ Flow cytometer instrument validation

    ✔ Multi-laboratory comparison

    ✔ Clinical protocol standardization

    Storage Conditions

    ❄ Store at 2–8 °C

    ❄ Protect from light

  • ❄ Do not freeze